Intermediate
Part:BBa_K2036007:Design
Designed by: Zhangyu Cheng Group: iGEM16_HUST-China (2016-09-19)
RBS-CII-RBS-CII, tandem expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
One can assemble this part right behind a promoter or at the uptsream of pRE. It can serve as a positive regulation of a certain gene downstream pRE. And according to the Registry: prefix of none coding sequence are supposed to be"GAATTCGCGGCCGCTTCTAGAG", so the scar between two subpart should actually be TACTAGAG but not TACTAG as doucumented in the sequence page.
Source
come from bacteraphage lambda